Ajla Ruzic of Croatia enters the London grass-court match ranked 64th globally. The Croatian player has shown mixed form in recent competition, with two wins against A. Krueger and K. Rakhimova offset by three losses to higher-ranked opponents M. Keys, Y. Putintseva, and M. Andreeva. This marks Ruzic's first career meeting with opponent A. Ito on grass courts.

Aika Ito of Japan is ranked 234th and faces Ruzic on unfamiliar grass in London. The Japanese player's recent record shows inconsistent results, including wins over A. Rajecki and J. Vandromme alongside losses to M. Sawangkaew, L. Sun, and L. Cabrera. With no prior head-to-head history against Ruzic, Ito will need to adapt quickly to the grass surface in this opening encounter.